WRITE ALL SEQUENCES 5’ TO 3’)1. Using the DNA sequence Below : (5 pts)5’-G

WRITE ALL SEQUENCES 5’ TO 3’)1. Using the DNA sequence Below : (5 pts)5’-GTCAGCATGCGACTGTTGCTGCAAAAATGACCATG-3’A. Write the complementary strand sequence:B. Design a set of primers (6 nucleotides long) for synthesizing the top and bottom strands. To synthesize the Top:To synthesize the Bottom:C. If the original strand is the coding strand for a gene, and transcription begins at the first “G” nucleotide, what would the RNA sequence look like?D. If the start of translation is the first AUG how many amino acids would this piece of DNA encode? E. What are the amino acids it would encode (write it N terminus to C terminus).Use any amino acid codon chart you want.2. Diagram the process by which eukaryotic mRNA is transcribed and processed to form the mature mRNA : (3 pt)3. The model below shows the regulation of a gene in E. coli. It is shown under four different conditions and the resulting rates of transcription are reported. Each “condition” in the model is a different food source for the bacteria. Fill in the following sugars in their corresponding blanks in the model if the gene in question codes for the β-galactosidase enzyme. (The enzyme that cleaves Lactose into Glucose and Galactose) (7pts)Condition A: Glucose and Lactose Condition C: Glycerol alone Condition B: Glucose alone Condition D: Lactose aloneCondition: A: _______________B: _______________C: _______________D: _______________sigmaAmount of transcript (compared to maximum)5%0.5%0.5%100%A + B are “transcription regulator proteins”RNA polymeraseABE. Why should a sugar source influence the production of β-galactosidase?F. Which transcription regulator is responding to the presence of glucose? _____ Explain your answer in one grammatically correct sentence.G. Which transcription regulator is responding to the presence of lactose? _____ Explain your answer in one grammatically correct sentence.